Proteintech, 14533-1-AP, LAMR1/RPSA Polyclonal antibody

Size: 20ul / 150ul The LAMR1/RPSA (14533-1-AP) by Proteintech is a Polyclonal antibody targeting LAMR1/RPSA in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 14533-1-AP targets LAMR1/RPSA in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: COLO 320 cells, mouse liver tissue, mouse colon tissue, HeLa cells, NIH/3T3 cells, PC-12 cells Positive IHC detected in: human stomach tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension Background Information The ribosomal protein SA (RPSA), previously named 67 kD laminin receptor(67LR), 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein, is a multifunctional protein, that plays a role in a number of pathological processes, such as cancer and prion diseases. It is overexpressed in various cancer cell lines, and the the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. This antibody is a rabbit polyclonal antibody raised against full length RPSA of human origin. This antibody is specific to RPSA (LAMR1).67LR derives from homo- or hetero- dimerization of a 37 kDa cytosolic precursor (37LRP)], most probably by fatty acid acylation. 37LRP is mostly found in the cytosol and nucleus [10] where it is involved in translational processes and maintenance of nuclear structures, respectively. 67LR is localized at the cell surface and it not only serves as a receptor for LM but also acts as a receptor for elastin, carbohydrates and the cellular prion protein. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, pig Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6033 Product name: Recombinant human LAMR1,RPSA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-295 aa of BC050688 Sequence: MSGALDVLQMKEEDVLKFLAAGTHLGGTNLDFQMEQYIYKRKSDGIYIINLKRTWEKLLLAARAIVAIENPADVSVISSRNTGQRAVLKFAAATGATPIAGRFTPGTFTNQIQATFREPRLLVVTDPRADHQPLTEASYVNLPTIALCNTDSPLRYVDIAIPCNNKGAHSVGLMWWMLAREVLRMRGTISREHPWEVMPDLYFYRDPEEIEKEEQAAAEKAVTKEEFQGEWTAPAPEFTATQPEVADWSEGVQVPSVPIQQFPTEDWSAQPATEDWSAAPTAQATEWVGATTDWS Predict reactive species Full Name: ribosomal protein SA Calculated Molecular Weight: 33 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: BC050688 Gene Symbol: RPSA Gene ID (NCBI): 3921 RRID: AB_2182528 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P08865 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.