Proteintech, 14572-1-AP, TFAP2C/AP2 gamma Polyclonal antibody

Size: 20ul / 150ul The TFAP2C/AP2 gamma (14572-1-AP) by Proteintech is a Polyclonal antibody targeting TFAP2C/AP2 gamma in WB, IHC, IP, ELISA applications with reactivity to human samples 14572-1-AP targets TFAP2C/AP2 gamma in WB, IHC, IP, chIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, human placenta tissue, MCF-7 cells Positive IP detected in: HeLa cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2400 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information TFAP2C (Transcription Factor AP-2 Gamma) is a member of the AP-2 transcription factor family, which plays a crucial role in early embryonic development, specifically in regulating the development of trophoblast cells and maintaining pluripotency. TFAP2C is known to regulate transcription by opening enhancers and is associated with a set of enhancers present in human embryos. It also functions as a regulator of both inner cell mass and trophectoderm differentiation, and controls the expression of cell-polarity genes. This protein binds to specific DNA sequences to activate or repress target genes, making it a key player in cellular processes and a potential therapeutic target in oncology research. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5648 Product name: Recombinant human TFAP2C protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-450 aa of BC035664 Sequence: MLWKITDNVKYEEDCEDRHDGSSNGNPRVPHLSSAGQHLYSPAPPLSHTGVAEYQPPPYFPPPYQQLAYSQSADPYSHLGEAYAAAINPLHQPAPTGSQQQAWPGRQSQEGAGLPSHHGRPAGLLPHLSGLEAGAVSARRDAYRRSDLLLPHAHALDAAGLAENLGLHDMPHQMDEVQNVDDQHLLLHDQTVIRKGPISMTKNPLNLPCQKELVGAVMNPTEVFCSVPGRLSLLSSTSKYKVTVAEVQRRLSPPECLNASLLGGVLRRAKSKNGGRSLREKLDKIGLNLPAGRRKAAHVTLLTSLVEGEAVHLARDFAYVCEAEFPSKPVAEYLTRPHLGGRNEMAARKNMLLAAQQLCKEFTELLSQDRTPHGTSRLAPVLETNIQNCLSHFSLITHGFGSQAICAAVSALQNYIKEALIVIDKSYMNPGDQSPADSNKTLEKMEKHRK Predict reactive species Full Name: transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma) Calculated Molecular Weight: 49 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC035664 Gene Symbol: TFAP2C Gene ID (NCBI): 7022 RRID: AB_2271424 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q92754 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.