Proteintech, 14578-1-AP, Pikachurin Polyclonal antibody

Size: 20ul / 150ul The Pikachurin (14578-1-AP) by Proteintech is a Polyclonal antibody targeting Pikachurin in WB, IF/ICC, IF-P, ELISA applications with reactivity to human, mouse, rat samples 14578-1-AP targets Pikachurin in WB, IF/ICC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human placenta tissue, PC-3 cells, SH-SY5Y cells Positive IF-P detected in: mouse eye tissue Positive IF/ICC detected in: SH-SY5Y cells, Y79 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Pikachurin, also known as EGFLAM or FLJ39155, is an extracellular matrix-like retinal protein that has been found to localize in the synaptic cleft of the photoreceptor ribbon synapse (PMID: 18641643). Pikachurin is a dystroglycan-interacting protein and has an essential role in photoreceptor ribbon synapse formation. Pikachurin is a 1017-amino-acid protein with a calculated molecular weight of 111 kDa and high mRNA abundance in retina. This antibody detects a 60-kDa band, which has been reported and likely represents a fragment resulting from cleavage of Pikachurin (PMID: 23226524). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5986 Product name: Recombinant human EGFLAM protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-152 aa of BC033177 Sequence: MRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNGSFNDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGMMRQLNINGALYVGGMKEIALHTNRQYMRGLVGCISHFTLSTDYHISLVEDSVDGKNINTCGAK Predict reactive species Full Name: EGF-like, fibronectin type III and laminin G domains Calculated Molecular Weight: 111 kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: BC033177 Gene Symbol: Pikachurin Gene ID (NCBI): 133584 RRID: AB_2277653 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q63HQ2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.