Proteintech, 14589-1-AP, IKIP Polyclonal antibody

Size: 20ul / 150ul The IKIP (14589-1-AP) by Proteintech is a Polyclonal antibody targeting IKIP in WB, IHC, ELISA applications with reactivity to human, mouse samples 14589-1-AP targets IKIP in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse skeletal muscle tissue, mouse spleen tissue, mouse thymus tissue Positive IHC detected in: human ovary tumor tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:100-1:400 Background Information IKIP(Inhibitor of nuclear factor kappa-B kinase-interacting protein) is also named as IKBIP and acts as a novel p53 target gene with proapoptotic function. A variety of experiments looking at endogenous IKIP1 levels showed that IKIP1 expression is dependent on p53. Transfection of IKIP into endothelial cells promotes cell death, as measured by TUNEL staining. EST analysis and RT-PCR demonstrated that 3 IKIP transcripts are generated; IKIP1 encodes a 377-amino acid protein, IKIP2 a 350-amino acid protein, and IKIP3 2 potential ORFs encoding 70- and 245-amino acid proteins(PMID:15389287). Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6119 Product name: Recombinant human IKIP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-377 aa of BC058933 Sequence: MSEVKSRKKSGPKGAPAAEPGKRSEGGKTPVARSSGGGGWADPRTCLSLLSLGTCLGLAWFVFQQSEKFAKVENQYQLLKLETNEFQQLQSKISLISEKLESTESILQEATSSMSLMTQFEQEVSNLQDIMHDIQNNEEVLTQRMQSLNEKFQNITDFWKRSLEEMNINTDIFKSEAKHIHSQVTVQINSAEQEIKLLTERLKDLEDSTLRNIRTVKRQEEEDLLRVEEQLGSDTKAIEKLEEEQHALFARDEDLTNKLSDYEPKVEECKTHLPTIESAIHSVLRVSQDLIETEKKMEDLTMQMFNMEDDMLKAVSEIMEMQKTLEGIQYDNSILKMQNELDILKEKVHDFIAYSSTGEKGTLKEYNIENKGIGGDF Predict reactive species Full Name: IKK interacting protein Calculated Molecular Weight: 39 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: BC058933 Gene Symbol: IKIP Gene ID (NCBI): 121457 RRID: AB_1557451 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q70UQ0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.