Proteintech, 14667-1-AP, Decorin Polyclonal antibody

Size: 20ul / 150ul The Decorin (14667-1-AP) by Proteintech is a Polyclonal antibody targeting Decorin in WB, IHC, IF/ICC, IF-P, ELISA applications with reactivity to human, mouse, rat, zabrafish samples 14667-1-AP targets Decorin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, zabrafish samples. Tested Applications Positive WB detected in: mouse heart tissue, MCF-7 cells, BxPC-3 cells, mouse colon tissue, rat heart tissue, NIH/3T3 cells Positive IHC detected in: human skin cancer tissue, human breast cancer tissue, human breast hyperplasia tissue, human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: zebrafish retina, mouse liver tissue Positive IF/ICC detected in: BxPC-3 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. The binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. Decorin(~ 40 kDa) also binds glycosaminoglycan chains to form big molecules. Specification Tested Reactivity: human, mouse, rat, zabrafish Cited Reactivity: human, mouse, rat, hamster Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6328 Product name: Recombinant human DCN protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-359 aa of BC005322 Sequence: MKATIILLLLAQVSWAGPFQQRGLFDFMLEDEASGIGPEVPDDRDFEPSLGPVCPFRCQCHLRVVQCSDLGLDKVPKDLPPDTTLLDLQNNKITEIKDGDFKNLKNLHALILVNNKISKVSPGAFTPLVKLERLYLSKNQLKELPEKMPKTLQELRAHENEITKVRKVTFNGLNQMIVIELGTNPLKSSGIENGAFQGMKKLSYIRIADTNITSIPQGLPPSLTELHLDGNKISRVDAASLKGLNNLAKLGLSFNSISAVDNGSLANTPHLRELHLDNNKLTRVPGGLAEHKYIQVVYLHNNNISVVGSSDFCPPGHNTKKASYSGVSLFSNPVQYWEIQPSTFRCVYVRSAIQLGNYK Predict reactive species Full Name: decorin Calculated Molecular Weight: 359 aa, 40 kDa Observed Molecular Weight: 70 kDa, 45-48 kDa GenBank Accession Number: BC005322 Gene Symbol: Decorin Gene ID (NCBI): 1634 RRID: AB_2090265 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P07585 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.