Proteintech, 14731-1-AP, TMEM98 Polyclonal antibody

Size: 20ul / 150ul The TMEM98 (14731-1-AP) by Proteintech is a Polyclonal antibody targeting TMEM98 in WB, IP, IHC, ELISA applications with reactivity to human, mouse samples 14731-1-AP targets TMEM98 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse E12.5 embryos Positive IP detected in: Y79 cells Positive IHC detected in: mouse embryo tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information TMEM98, also named as TADA1, composed of 226 amino acid residues, is a potential single-pass transmembrane protein. It is expression in both nucleus and cytoplasm. TMEM98 was found to be over-expressed in most of the hepatocellular carcinoma tissue. MEM98 is not only a prognostic marker for patients with hepatocellular carcinoma but also a novel molecular target associated with intrinsic and acquired chemoresistance of hepatocellular carcinoma (PMID: 24608572). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6492 Product name: Recombinant human TMEM98 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 27-226 aa of BC000526 Sequence: CRQRYCRPRDLLQRYDSKPIVDLIGAMETQSEPSELELDDVVITNPHIEAILENEDWIEDASGLMSHCIAILKICHTLTEKLVAMTMGSGAKMKTSASVSDIIVVAKRISPRVDDVVKSMYPPLDPKLLDARTTALLLSVSHLVLVTRNACHLTGGLDWIDQSLSAAEEHLEVLREAALASEPDKGLPGPEGFLQEQSAI Predict reactive species Full Name: transmembrane protein 98 Calculated Molecular Weight: 25 kDa Observed Molecular Weight: 21-25 kDa GenBank Accession Number: BC000526 Gene Symbol: TMEM98 Gene ID (NCBI): 26022 RRID: AB_11182589 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y2Y6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.