Proteintech, 14761-1-AP, WRAP53 Polyclonal antibody

Size: 20ul / 150ul The WRAP53 (14761-1-AP) by Proteintech is a Polyclonal antibody targeting WRAP53 in WB, IP, ELISA applications with reactivity to human samples 14761-1-AP targets WRAP53 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Raji cells, human kidney tissue, HeLa cells Positive IP detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information WRAP53 (WD40-encoding RNA Antisense to p53), also known as TCAB1 and WDR79, is a multifunctional protein encoded by a gene that produces both a coding mRNA and a regulatory antisense RNA targeting the tumor suppressor p53. Initially identified for its role in maintaining genomic stability by regulating p53 expression, WRAP53 is crucial for telomerase assembly and localization to Cajal bodies, impacting telomere maintenance. It also participates in DNA damage repair and cellular stress responses. The calculated molecular weight of WRAP53 is about 59 kDa, but the actual observed molecular weight is about 72-75 kDa, which may be due to modification. Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6522 Product name: Recombinant human WRAP53 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 166-548 aa of BC002336 Sequence: TQPENFLKGCKWAPDGSCILTNSADNILRIYNLPPELYHEGEQVEYAEMVPVLRMVEGDTIYDYCWYSLMSSAQPDTSYVASSSRENPIHIWDAFTGELRASFRAYNHLDELTAAHSLCFSPDGSQLFCGFNRTVRVFSTARPGRDCEVRATFAKKQGQSGIISCIAFSPAQPLYACGSYGRSLGLYAWDDGSPLALLGGHQGGITHLCFHPDGNRFFSGARKDAELLCWDLRQSGYPLWSLGREVTTNQRIYFDLDPTGQFLVSGSTSGAVSVWDTDGPGNDGKPEPVLSFLPQKDCTNGVSLHPSLPLLATASGQRVFPEPTESGDEGEELGLPLLSTRHVHLECRLQLWWCGGAPDSSIPDDHQGEKGQGGTEGGVGELI Predict reactive species Full Name: WD repeat containing, antisense to TP53 Calculated Molecular Weight: 59 kDa Observed Molecular Weight: 72-75 kDa GenBank Accession Number: BC002336 Gene Symbol: WRAP53 Gene ID (NCBI): 55135 RRID: AB_1939806 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BUR4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.