Proteintech, 14809-1-AP, OBFC2B Polyclonal antibody

Size: 20ul / 150ul OBFC2B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA 14809-1-AP targets OBFC2B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, NIH/3T3 cells, HeLa cells Positive IP detected in: HeLa cells Positive IHC detected in: human ovary tumor tissue, human pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: Hela cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information OBFC2B, also named as NABP2 or SSB1, is a 211 amino acid protein, which belongs to the SOSS-B family. OBFC2B localizes to nuclear foci following DNA damage. OBFC2B is a component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. In the SOSS complex, OBFC2B acts as a sensor of single-stranded DNA that binds to single-stranded DNA, in particular to polypyrimidines. The calculated molecular weight of OBFC2B is 22 kDa, but modified OBFC2B is about 32 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6532 Product name: Recombinant human OBFC2B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-211 aa of BC001079 Sequence: MTTETFVKDIKPGLKNLNLIFIVLETGRVTKTKDGHEVRTCKVADKTGSINISVWDDVGNLIQPGDIIRLTKGYASVFKGCLTLYTGRGGDLQKIGEFCMVYSEVPNFSEPNPEYSTQQAPNKAVQNDSNPSASQPTTGPSAASPASENQNGNGLSAPPGPGGGPHPPHTPSHPPSTRITRSQPNHTPAGPPGPSSNPVSNGKETRRSSKR Predict reactive species Full Name: oligonucleotide/oligosaccharide-binding fold containing 2B Calculated Molecular Weight: 22 kDa Observed Molecular Weight: 30-33 kDa GenBank Accession Number: BC001079 Gene Symbol: OBFC2B Gene ID (NCBI): 79035 RRID: AB_2251673 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BQ15 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.