Proteintech, 14844-1-AP, Myh9 Polyclonal antibody

Size: 20ul / 150ul The Myh9 (14844-1-AP) by Proteintech is a Polyclonal antibody targeting Myh9 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, canine samples 14844-1-AP targets Myh9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, canine samples. Tested Applications Positive WB detected in: A549 cells, HeLa cells, SGC-7901 cells Positive IHC detected in: mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MDCK cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information MYH9, also named as NMMHC-A and NMMHC-IIA, is a cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. It plays an important role in cytoskeleton reorganization, focal contacts formation (in the margins but not the central part of spreading cells), and lamellipodial retraction, during cell spreading. Specification Tested Reactivity: human, mouse, canine Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: myosin, heavy polypeptide 9, non-muscle Calculated Molecular Weight: 226 kDa Observed Molecular Weight: 224 kDa GenBank Accession Number: NM_022410 Gene Symbol: Myh9 Gene ID (NCBI): 17886 RRID: AB_2147311 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8VDD5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.