Proteintech, 14845-1-AP, CRLS1-Specific Polyclonal antibody

Size: 20ul / 150ul The CRLS1-Specific (14845-1-AP) by Proteintech is a Polyclonal antibody targeting CRLS1-Specific in WB, IHC, ELISA applications with reactivity to human samples 14845-1-AP targets CRLS1-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, HEK-293T cells, HepG2 cells, Jurkat cells Positive IHC detected in: human hepatocellular carcinomaNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Cardiolipin (CL) is a mitochondrial-specific double negatively charged phospholipid which is intricately involved in regulating bioenergetic efficiency. In eukaryotes, CL is synthesized by cardiolipin synthase (CRLS1 or CLS1) which catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form CL and glycerol. Recently it has been reported that transgenic expression of CRLS1 accelerates cardiolipin remodeling, improves mitochondrial function, modulates mitochondrial signaling, and attenuates mitochondrial dysfunction during diabetes, thereby identifying CRLS1 as a novel therapeutic target to attenuate mitochondrial dysfunction in diabetic myocardium. The predicted molecular weight of CRLS1 is around 32-35 kDa, while a 50 kDa protein with CRLS1 activity had been observed in liver isolation, which may represent an isoform of CRLS1 (20652826). Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: cardiolipin synthase 1 Calculated Molecular Weight: 33 kDa Observed Molecular Weight: 32 kDa GenBank Accession Number: NM_019095 Gene Symbol: CRLS1 Gene ID (NCBI): 54675 RRID: AB_2085336 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UJA2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.