Proteintech, 14855-1-AP, RD3 Polyclonal antibody

Size: 20ul / 150ul The RD3 (14855-1-AP) by Proteintech is a Polyclonal antibody targeting RD3 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 14855-1-AP targets RD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse retina tissue, rat retina tissue Positive IHC detected in: rat eye tissue, mouse eye tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: H9C2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information RD3, or Retinal Degeneration 3, plays a critical role in regulating guanylate cyclase (GC) signaling and photoreceptor cell survival (PMID: 30559291). RD3 is highly conserved across vertebrates, with the human protein sharing high sequence identity with other primates and varying degrees of identity with other species (PMID: 29030614). The main functions of RD3 include inhibiting photoreceptor-specific guanylate cyclase activity and promoting the accumulation of retinal membrane guanylyl cyclase (RetGC) in the photoreceptor outer segment (PMID: 30559291). RD3 is essential for the normal expression of RetGC in photoreceptor cells and blocks RetGC catalytic activity. Mutations in the RD3 gene can lead to Leber congenital amaurosis type 12, which results in retinal degeneration. RD3 is also involved in the trafficking of RetGC from the endoplasmic reticulum to the photoreceptor outer segments, which is crucial for maintaining the normal function and survival of photoreceptors (PMID: 34537244). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6641 Product name: Recombinant human RD3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-195 aa of BC065541 Sequence: MSLISWLRWNEAPSRLSTRSPAEMVLETLMMELTGQMREAERQQRERSNAVRKVCTGVDYSWLASTPRSTYDLSPIERLQLEDVCVKIHPSYCGPAILRFRQLLAEQEPEVQEVSQLFRSVLQEVLERMKQEEEAHKLTRQWSLRPRGSLATFKTRARISPFASDIRTISEDVERDTPPPLRSWSMPEFRAPKAD Predict reactive species Full Name: retinal degeneration 3 Calculated Molecular Weight: 22.7 kDa Observed Molecular Weight: 23 kDa GenBank Accession Number: BC065541 Gene Symbol: RD3 Gene ID (NCBI): 343035 RRID: AB_3085443 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q7Z3Z2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.