Proteintech, 14865-1-AP, SDHA Polyclonal antibody

Size: 20ul / 150ul The SDHA (14865-1-AP) by Proteintech is a Polyclonal antibody targeting SDHA in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat, pig, zebrafish samples 14865-1-AP targets SDHA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig, zebrafish samples. Tested Applications Positive WB detected in: HEK-293 cells, pig brain tissue, HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue, zebrafish Positive IP detected in: HEK-293 cells Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information SDHA, also named as SDH2, SDHF and Fp, belongs to the FAD-dependent oxidoreductase 2 family and FRD/SDH subfamily. SDHA is the flavoprotein subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDHA has 3 isoforms with the molecular mass of 73, 67 and 57 kDa, and the N-terminal (~5 kDa) can be removed in mature form. It can be detected a band of 68 kDa in cutaneous melanoma(PMID:21698147). SDHA may act as a tumor suppressor. Specification Tested Reactivity: human, mouse, rat, pig, zebrafish Cited Reactivity: human, mouse, rat, pig, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6677 Product name: Recombinant human SDHA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 336-664 aa of BC001380 Sequence: DLASRDVVSRSMTLEIREGRGCGPEKDHVYLQLHHLPPEQLATRLPGISETAMIFAGVDVTKEPIPVLPTVHYNMGGIPTNYKGQVLRHVNGQDQIVPGLYACGEAACASVHGANRLGANSLLDLVVFGRACALSIEESCRPGDKVPPIKPNAGEESVMNLDKLRFADGSIRTSELRLSMQKSMQNHAAVFRVGSVLQEGCGKISKLYGDLKHLKTFDRGMVWNTDLVETLELQNLMLCALQTIYGAEARKESRGAHAREDYKVRIDEYDYSKPIQGQQKKPFEEHWRKHTLSYVDVGTGKVTLEYRPVIDKTLNEADCATVPPAIRSY Predict reactive species Full Name: succinate dehydrogenase complex, subunit A, flavoprotein (Fp) Calculated Molecular Weight: 73 kDa Observed Molecular Weight: 62-73 kDa GenBank Accession Number: BC001380 Gene Symbol: SDHA Gene ID (NCBI): 6389 RRID: AB_11182164 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P31040 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.