Proteintech, 14876-1-AP, DAAM1 Polyclonal antibody

Size: 20ul / 150ul The DAAM1 (14876-1-AP) by Proteintech is a Polyclonal antibody targeting DAAM1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 14876-1-AP targets DAAM1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, HepG2 cells, human heart tissue, mouse liver tissue, mouse heart tissue Positive IP detected in: mouse liver tissue Positive IHC detected in: human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information Dishevelled-associated activator of morphogenesis 1 (Daam1) is a member of the formin protein family that is implicated in actin assembly. Daam1 also plays a critical role in mediating Wnt/Fz signaling pathway and regulates cell polarity during development and tissue homoeostasis. This antibody well recognizes the endogenous DAAM1 protein around 120 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, canine, goat, xenopus Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6648 Product name: Recombinant human DAAM1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 715-1068 aa of BC064999 Sequence: KDMLEQLLKFVPEKSDIDLLEEHKHELDRMAKADRFLFEMSRINHYQQRLQSLYFKKKFAERVAEVKPKVEAIRSGSEEVFRSGALKQLLEVVLAFGNYMNKGQRGNAYGFKISSLNKIADTKSSIDKNITLLHYLITIVENKYPSVLNLNEELRDIPQAAKVNMTELDKEISTLRSGLKAVETELEYQKSQPPQPGDKFVSVVSQFITVASFSFSDVEDLLAEAKDLFTKAVKHFGEEAGKIQPDEFFGIFDQFLQAVSEAKQENENMRKKKEEEERRARMEAQLKEQRERERKMRKAKENSEESGEFDDLVSALRSGEVFDKDLSKLKRNRKRITNQMTDSSRERPITKLNF Predict reactive species Full Name: dishevelled associated activator of morphogenesis 1 Calculated Molecular Weight: 123 kDa Observed Molecular Weight: 123 kDa GenBank Accession Number: BC064999 Gene Symbol: DAAM1 Gene ID (NCBI): 23002 RRID: AB_2089444 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y4D1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.