Proteintech, 14909-1-AP, RPS26 Polyclonal antibody

Size: 20ul / 150ul The RPS26 (14909-1-AP) by Proteintech is a Polyclonal antibody targeting RPS26 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 14909-1-AP targets RPS26 in WB, IHC, IF/ICC, IP, ELISA, PLA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse ovary tissue, mouse lung tissue Positive IP detected in: mouse lung tissue Positive IHC detected in: human breast cancer tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information Diamond-Blackfan anemia (DBA) is a congenital red cell aplasia that is usually diagnosed during early infancy, and strikingly, in addition to defects in red cell maturation the disorder is associated with various physical anomalies in about 40% of patients. To date, heterozygous mutations in nine RP genes have been conclusively associated with DBA, and RPS26 is one of them. There are nine mutation found in RPS26 from DBA patients. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6706 Product name: Recombinant human RPS26 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-115 aa of BC002604 Sequence: MTKKRRNNGRAKKGRGHVQPIRCTNCARCVPKDKAIKKFVIRNIVEAAAVRDISEASVFDAYVLPKLYVKLHYCVSCAIHSKVVRNRSREARKDRTPPPRFRPAGAAPRPPPKPM Predict reactive species Full Name: ribosomal protein S26 Calculated Molecular Weight: 13 kDa Observed Molecular Weight: 18-21 kDa GenBank Accession Number: BC002604 Gene Symbol: RPS26 Gene ID (NCBI): 6231 RRID: AB_2180361 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P62854 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.