Proteintech, 14992-1-AP, HIRIP3 Polyclonal antibody

Size: 20ul / 150ul The HIRIP3 (14992-1-AP) by Proteintech is a Polyclonal antibody targeting HIRIP3 in WB, IHC, IP, ELISA applications with reactivity to human samples 14992-1-AP targets HIRIP3 in WB, IHC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, human heart tissue, human skeletal muscle tissue Positive IP detected in: HEK-293 cells Positive IHC detected in: human ovary tissue, human heart tissue, human kidney tissue, human placenta tissue, human skin tissue, human stomach cancer tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information The HIRIP3 protein had been identified from its interaction with the HIRA histone chaperone. It may play a role in chromatin function and histone metabolism via its interaction with HIRA and histones. Specification Tested Reactivity: human Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6978 Product name: Recombinant human HIRIP3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 207-556 aa of BC000588 Sequence: TAKKVEGNKGTKSLKESEQESEEEILAQKKEQREEEVEEEEKEEDEEKGDWKPRTRSNGRRKSAREERSCKQKSQAKRLLGDSDSEEEQKEAASSGDDSGRDREPPVQRKSEDRTQLKGGKRLSGSSEDEEDSGKGEPTAKGSRKMARLGSTSGEESDLEREVSDSEAGGGPQGERKNRSSKKSSRKGRTRSSSSSSDGSPEAKGGKAGSGRRGEDHPAVMRLKRYIRACGAHRNYKKLLGSCCSHKERLSILRAELEALGMKGTPSLGKCRALKEQREEAAEVASLDVANIISGSGRPRRRTAWNPLGEAAPPGELYRRTLDSDEERPRPAPPDWSHMRGIISSDGESN Predict reactive species Full Name: HIRA interacting protein 3 Calculated Molecular Weight: 62 kDa Observed Molecular Weight: 69-90 kDa GenBank Accession Number: BC000588 Gene Symbol: HIRIP3 Gene ID (NCBI): 8479 RRID: AB_2117876 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BW71 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.