Proteintech, 15016-1-AP, DNPEP Polyclonal antibody

Size: 20ul / 150ul The DNPEP (15016-1-AP) by Proteintech is a Polyclonal antibody targeting DNPEP in WB, IF/ICC, IP, ELISA applications with reactivity to human samples 15016-1-AP targets DNPEP in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, MCF-7 cells, K-562 cells Positive IP detected in: HEK-293 cells Positive IF/ICC detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information DNPEP, also named as ASPEP and DAP, plays an important role in intracellular protein and peptide metabolism. It catalyzes the hydrolysis of N-terminal aspartyl and glutamyl residues. Specification Tested Reactivity: human Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag6966 Product name: Recombinant human DNPEP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 130-475 aa of BC000653 Sequence: FDRDLTLAGRVIVKCPTSGRLEQQLVHVERPILRIPHLAIHLQRNINENFGPNTEMHLVPILATAIQEELEKGTPEPGPLNAVDERHHSVLMSLLCAHLGLSPKDIVEMELCLADTQPAVLGGAYDEFIFAPRLDNLHSCFCALQALIDSCAGPGSLATEPHVRMVTLYDNEEVGSESAQGAQSLLTELVLRRISASCQHPTAFEEAIPKSFMISADMAHAVHPNYLDKHEENHRPLFHKGPVIKVNSKQRYASNAVSEALIREVANKVKVPLQDLMVRNDTPCGTTIGPILASRLGLRVLDLGSPQLAMHSIREMACTTGVLQTLTLFKGFFELFPSLSHNLLVD Predict reactive species Full Name: aspartyl aminopeptidase Calculated Molecular Weight: 52 kDa Observed Molecular Weight: 55 kDa GenBank Accession Number: BC000653 Gene Symbol: DNPEP Gene ID (NCBI): 23549 RRID: AB_2094293 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9ULA0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.