Proteintech, 15072-1-AP, TTBK2 Polyclonal antibody

Size: 20ul / 150ul The TTBK2 (15072-1-AP) by Proteintech is a Polyclonal antibody targeting TTBK2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 15072-1-AP targets TTBK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: PC-3 cells, HeLa cells, SH-SY5Y cells Positive IHC detected in: mouse brain tissue, human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: hTERT-RPE1 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information TTBK2(tau tubulin kinase 2) plays an important role in the tau cascade and in spinocerebellar degeneration. The N-terminus of TTBK2 has a serine-threonine-tyrosine kinase domain, and a C-terminal region shows homology to TTBK1. TTBK1 and TTBK2 function as regulators of TDP-43 phosphorylation. TTBK1/2 may be attractive drug targets for therapeutic interventions in TDP-43 proteinopathies such as FTLD-TDP and ALS.(PMID:25473830). 15072-1-AP has a cross reaction with TTBK1. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7108 Product name: Recombinant human TTBK2 protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 1-354 aa of BC071556 Sequence: MSGGGEQPDILSVGILVKERWKVLRKIGGGGFGEIYDALDMLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNDRFNYVVMQLQGRNLADLRRSQSRGTFTISTTLRLGRQILESIESIHSVGFLHRDIKPSNFAMGRFPSTCRKCYMLDFGLARQFTNSCGDVRPPRAVAGFRGTVRYASINAHRNREMGRHDDLWSLFYMLVEFVVGQLPWRKIKDKEQVGSIKERYDHRLMLKHLPPEFSIFLDHISSLDYFTKPDYQLLTSVFDNSIKTFGVIESDPFDWEKTGNDGSLTTTTTSTTPQLHTRLTPAAIGIANATPIPGDLLRENTDEVFPDEQLSD Predict reactive species Full Name: tau tubulin kinase 2 Calculated Molecular Weight: 137 kDa Observed Molecular Weight: 137-150 kDa GenBank Accession Number: BC071556 Gene Symbol: TTBK2 Gene ID (NCBI): 146057 RRID: AB_2211507 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6IQ55 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.