Proteintech, 15082-1-AP, LPCAT2 Polyclonal antibody

Size: 20ul / 150ul The LPCAT2 (15082-1-AP) by Proteintech is a Polyclonal antibody targeting LPCAT2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 15082-1-AP targets LPCAT2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, LPS treated HEK-293, HeLa cells, Jurkat cells, RAW 264.7 cells Positive IP detected in: HeLa cells Positive IHC detected in: rat thyroid gland tissue, human rectal cancer tissue, mouse brain tissue, mouse small intestine tissue, rat small intestine tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7156 Product name: Recombinant human LPCAT2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 161-544 aa of BC002472 Sequence: PSMVSRNENAQVPLIGRLLRAVQPVLVSRVDPDSRKNTINEIIKRTTSGGEWPQILVFPEGTCTNRSCLITFKPGAFIPGVPVQPVLLRYPNKLDTVTWTWQGYTFIQLCMLTFCQLFTKVEVEFMPVQVPNDEEKNDPVLFANKVRNLMAEALGIPVTDHTYEDCRLMISAGQLTLPMEAGLVEFTKISRKLKLDWDGVRKHLDEYASIASSSKGGRIGIEEFAKYLKLPVSDVLRQLFALFDRNHDGSIDFREYVIGLAVLCNPSNTEEIIQVAFKLFDVDEDGYITEEEFSTILQASLGVPDLDVSGLFKEIAQGDSISYEEFKSFALKHPEYAKIFTTYLDLQTCHVFSLPKEVQTTPSTASNKVSPEKHEESTSDKKDD Predict reactive species Full Name: lysophosphatidylcholine acyltransferase 2 Calculated Molecular Weight: 60 kDa Observed Molecular Weight: 53 kDa GenBank Accession Number: BC002472 Gene Symbol: LPCAT2 Gene ID (NCBI): 54947 RRID: AB_2135557 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q7L5N7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.