Proteintech, 15112-1-AP, CAPRIN1 Polyclonal antibody

Size: 20ul / 150ul The CAPRIN1 (15112-1-AP) by Proteintech is a Polyclonal antibody targeting CAPRIN1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples 15112-1-AP targets CAPRIN1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, mouse brain tissue, HEK-293T cells, HeLa cells, NIH/3T3 cells, rat brain tissue, Jurkat cells Positive IP detected in: HeLa cells Positive IHC detected in: human breast cancer tissue, human prostate cancer tissue, human testis tissue, mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells, sodium arsenite treated HeLa cells, HepG2 cells, OC2 cells, cochlear cryosections Positive FC (Intra) detected in: NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension Background Information CAPRIN1 (Caprin-1) is a cytoplasmic phosphoprotein which may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Its levels, except in the brain, are tightly correlated with cellular proliferation. Suppression of expression of human Caprin-1 resulted in slowing of the proliferation rate,which suggests its essential for normal cellular proliferation. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, pig, rabbit, canine, monkey, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7099 Product name: Recombinant human CAPRIN1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 356-709 aa of BC001731 Sequence: RQRVQDLMAQMQGPYNFIQDSMLDFENQTLDPAIVSAQPMNPTQNMDMPQLVCPPVHSESRLAQPNQVPVQPEATQVPLVSSTSEGYTASQPLYQPSHATEQRPQKEPIDQIQATISLNTDQTTASSSLPAASQPQVFQAGTSKPLHSSGINVNAAPFQSMQTVFNMNAPVPPVNEPETLKQQNQYQASYNQSFSSQPHQVEQTELQQEQLQTVVGTYHGSPDQSHQVTGNHQQPPQQNTGFPRSNQPYYNSRGVSRGGSRGARGLMNGYRGPANGFRGGYDGYRPSFSNTPNSGYTQSQFSAPRDYSGYQRDGYQQNFKRGSGQSGPRGAPRGRGGPPRPNRGMPQMNTQQVN Predict reactive species Full Name: cell cycle associated protein 1 Calculated Molecular Weight: 78 kDa Observed Molecular Weight: 116 kDa GenBank Accession Number: BC001731 Gene Symbol: CAPRIN1 Gene ID (NCBI): 4076 RRID: AB_2070016 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q14444 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.