Proteintech, 15147-1-AP, CEP68 Polyclonal antibody

Size: 20ul / 150ul The CEP68 (15147-1-AP) by Proteintech is a Polyclonal antibody targeting CEP68 in WB, ELISA applications with reactivity to human samples 15147-1-AP targets CEP68 in WB, IF, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: SH-SY5Y cells, HeLa cells, Jurkat cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Background Information CEP68, or centrosomal protein 68, is a protein-coding gene in humans. It is involved in the maintenance of centrosome cohesion, likely as part of a linker structure that prevents centrosome splitting. CEP68 is also required for the localization of CDK5RAP2 to the centrosome during interphase and contributes to the formation of CROCC/rootletin filaments. This protein is encoded by the CEP68 gene and is located on the fibers emanating from the proximal ends of centrioles. It has been associated with diseases such as Retinitis Pigmentosa 28 and is implicated in angioedema. The gene is mapped to chromosome 2 and is expressed ubiquitously in various tissues, including the brain, ovary, and others. The protein plays a crucial role in cellular functions related to the centrosome, which is a key organelle in cell division and organization. This antibody recognized two bands of ∼90 and 67 kDa, respectively, in good agreement with the predicted sizes of the two Cep68 isoforms (81 and 67 kDa for Cep68L and -S, respectively). Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7447 Product name: Recombinant human CEP68 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC002982 Sequence: MALGEEKAEAEASEDTKAQSYGRGSCRERELDIPGPMSGEQPPRLEAEGGLISPVWGAEGIPAPTCWIGTDPGSPSRAHQPQASDANREPVAERSEPALSGLPPATMGSGDLLLSGESQVEKTKLSSSEEFPQTLSLPRTTTICSGHDADTEDDPSLADLPQALDLSQQPHSSGLSCLSQWKSVLSPGSAAQPSSCSISASSTGSSLQGHQERAEPRGGSLAKVSSSLEPVVPQEPSSVVGLGPRPQWSPQPVFSGGDASGLGRRRLSFQAEYWACVLPDSLPPSPDRHSPLWNPNKEYEDLLDYTYPLRPGPQLPKHLDSRVPADPVLQDSGVDLDSFSVSPASTLKSPT Predict reactive species Full Name: centrosomal protein 68kDa Calculated Molecular Weight: 81 kDa Observed Molecular Weight: 90 kDa, 67 kDa GenBank Accession Number: BC002982 Gene Symbol: CEP68 Gene ID (NCBI): 23177 RRID: AB_2077081 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q76N32 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.