Proteintech, 15255-1-AP, H6PD Polyclonal antibody

Size: 20ul / 150ul The H6PD (15255-1-AP) by Proteintech is a Polyclonal antibody targeting H6PD in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples 15255-1-AP targets H6PD in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, mouse liver tissue, HeLa cells Positive IP detected in: HepG2 cells Positive IHC detected in: human hepatocirrhosis tissue, human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information Hexose-6-phosphate dehydrogenase (H6PD) is also named as GDH,6PGL. It is the main NADPH generating enzyme in the lumen of the endoplasmic reticulum. H6PD is regarded as an ancillary enzyme in prereceptorial glucocorticoid activation and probably acts as a nutrient sensor and as a prosurvival factor(PMID:21620971). H6PD is a glycoprotein and has been shown tissue differences in glycosylation status might explain both the difference in migration on SDS-PAGE gels as well as in activity. Defects in H6PD are a cause of cortisone reductase deficiency (CRD). This antibody is specific to H6PD. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7109 Product name: Recombinant human H6PD protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 443-791 aa of BC081559 Sequence: LSDYYAYSPVQERDAHSVLLSHIFHGRKNFFITTENLLASWNFWTPLLESLAHKAPRLYPGGAENGRLLDFEFSSGRLFFSQQQPEQLVPGPGPAPMPSDFQVLRAKYRESPLVSAWSEELISKLANDIEATAVRAVRRFGQFHLALSGGSSPVALFQQLATAHYGFPWAHTHLWLVDERCVPLSDPESNFQGLQAHLLQHVRIPYYNIHPMPVHLQQRLCAEEDQGAQIYAREISALVANSSFDLVLLGMGADGHTASLFPQSPTGLDGEQLVVLTTSPSQPHRRMSLSLPLINRAKKVAVLVMGRMKREITTLVSRVGHEPKKWPISGVLPHSGQLVWYMDYDAFLG Predict reactive species Full Name: hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase) Calculated Molecular Weight: 89 kDa Observed Molecular Weight: 89-95 kDa GenBank Accession Number: BC081559 Gene Symbol: H6PD Gene ID (NCBI): 9563 RRID: AB_10642699 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O95479 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.