Proteintech, 15303-1-AP, GBP1 Polyclonal antibody

Size: 20ul / 150ul The GBP1 (15303-1-AP) by Proteintech is a Polyclonal antibody targeting GBP1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 15303-1-AP targets GBP1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human placenta tissue, human brain tissue, HUVEC cells Positive IP detected in: human placenta tissue Positive IHC detected in: human spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information Guanylate binding protein 1 (GBP1) belongs to the dynamin superfamily of large GTPases. The expression of GBP1 is induced by IFN and GBP1 is characterized by its ability to specifically bind guanine nucleotides such as GMP, GDP, and GTP and its ability to hydrolyze GTP to GDP and GMP.What is the molecular weight of GBP1?The molecular weight of GBP1 is 68kDa.What is the cellular localization of GBP1?GBP1 is predominantly cytosolic, but under certain conditions is farnesylated and associated with intracellular membranes such as the Golgi (PMID: 15937107).What is the role of GBP1 in immunity?GBP1 is induced in response to type I and type II IFN and as such plays a role in protective immunity against a spectrum of intracellular pathogens ranging from viruses to bacteria to protozoa, such as negative-strand RNA Rhabdovirus, vesicular stomatitis virus and the positive-strand RNA Picornovirus, encephalomyocarditis virus in cultured cells, the inhibition of Chlamydia trachomatis, Toxoplasma gondii, and Salmonella enterica (PMID: 21142871). These anti-pathogenic functions involve the recruitment to vacuolar compartments for the destruction of the pathogen, the assembly of innate immune complexes such as the inflammasome, or the initiation of autophagy.What is the role of GBP-1 in colorectal carcinoma?In colorectal carcinoma (CRC), the presence of tumor-infiltrating T helper type 1 (Th1) cells participates in anti-tumoral responses and an improved clinical outcome (PMID: 17008531, 20927778). IFN promote the Th1 immune response, as well as GBP-1 expression. In CRC, GBP-1 expression acts as a tumor suppressor, where inhibition of tumor growth by GBP-1 is the result of its strong antiangiogenic activity as well as its direct anti-tumorigenic effect on tumor cells and is correlated with a better outcome (PMID: 23042300). Specification Tested Reactivity: human Cited Reactivity: human, mouse, pig, chicken Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7562 Product name: Recombinant human GBP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 242-592 aa of BC002666 Sequence: VHRRKLAQLEKLQDEELDPEFVQQVADFCSYIFSNSKTKTLSGGIQVNGPRLESLVLTYVNAISSGDLPCMENAVLALAQIENSAAVQKAIAHYEQQMGQKVQLPTESLQELLDLHRDSEREAIEVFIRSSFKDVDHLFQKELAAQLEKKRDDFCKQNQEASSDRCSGLLQVIFSPLEEEVKAGIYSKPGGYRLFVQKLQDLKKKYYEEPRKGIQAEEILQTYLKSKESMTDAILQTDQTLTEKEKEIEVERVKAESAQASAKMLQEMQRKNEQMMEQKERSYQEHLKQLTEKMENDRVQLLKEQERTLALKLQEQEQLLKEGFQKESRIMKNEIQDLQTKMRRRKACTIS Predict reactive species Full Name: GTP binding protein 1 Calculated Molecular Weight: 68 kDa Observed Molecular Weight: 67 kDa GenBank Accession Number: BC002666 Gene Symbol: GBP1 Gene ID (NCBI): 2633 RRID: AB_2247448 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P32455 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.