Proteintech, 15437-1-AP, EPB41L2 Polyclonal antibody

Size: 20ul / 150ul The EPB41L2 (15437-1-AP) by Proteintech is a Polyclonal antibody targeting EPB41L2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 15437-1-AP targets EPB41L2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, Jurkat cells Positive IP detected in: HEK-293 cells Positive IHC detected in: human ovary tumor tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information EPB41L2, also known as 4.1G, is a member of the protein 4.1 family, which generally serves as adaptors linking transmembrane proteins to the cytoskeleton(PMID: 21482674). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag3647 Product name: Recombinant human EPB41L2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 334-633 aa of BC034718 Sequence: ALLGSYTLQAELGDYDPEEHGSIDLSEFQFAPTQTKELEEKVAELHKTHRGLSPAQADSQFLENAKRLSMYGVDLHHAKDSEGVDIKLGVCANGLLIYKDRLRINRFAWPKILKISYKRSNFYIKVRPAELEQFESTIGFKLPNHRAAKRLWKVCVEHHTFYRLVSPEQPPKAKFLTLGSKFRYSGRTQAQTRQASTLIDRPAPHFERTSSKRVSRSLDGAPIGVMDQSLMKDFPGAAGEISAYGPGLVSIAVVQDGDGRREVRSPTKAPHLQLIEGKSSHETLNIVEEKSGQRLGKTKE Predict reactive species Full Name: erythrocyte membrane protein band 4.1-like 2 Calculated Molecular Weight: 112 kDa Observed Molecular Weight: 130-150 kDa GenBank Accession Number: BC034718 Gene Symbol: EPB41L2 Gene ID (NCBI): 2037 RRID: AB_10951110 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O43491 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.