Proteintech, 15453-1-AP, SAR1B Polyclonal antibody

Size: 20ul / 150ul The SAR1B (15453-1-AP) by Proteintech is a Polyclonal antibody targeting SAR1B in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 15453-1-AP targets SAR1B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: L02 cells, HeLa cells, human liver tissue, mouse brain tissue, mouse liver tissue, rat liver tissue Positive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information SAR1B, also named as SARA2, SARB and GTBPB, belongs to the small GTPase superfamily and SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus. SAR1B is activated by the guanine nucleotide exchange factor PREB. It is involved in the selection of the protein cargo and the assembly of the COPII coat complex. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7718 Product name: Recombinant human SAR1B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-198 aa of BC002847 Sequence: MSFIFDWIYSGFSSVLQFLGLYKKTGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHVQARRVWKNYLPAINGIVFLVDCADHERLLESKEELDSLMTDETIANVPILILGNKIDRPEAISEERLREMFGLYGQTTGKGSISLKELNARPLEVFMCSVLKRQGYGEGFRWMAQYID Predict reactive species Full Name: SAR1 homolog B (S. cerevisiae) Calculated Molecular Weight: 22 kDa Observed Molecular Weight: 25-27 kDa GenBank Accession Number: BC002847 Gene Symbol: SAR1B Gene ID (NCBI): 51128 RRID: AB_2183985 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y6B6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.