Proteintech, 15624-1-AP, U2AF2/U2AF65 Polyclonal antibody

Size: 20ul / 150ul The U2AF2/U2AF65 (15624-1-AP) by Proteintech is a Polyclonal antibody targeting U2AF2/U2AF65 in WB, IHC, ELISA applications with reactivity to human samples 15624-1-AP targets U2AF2/U2AF65 in WB, IHC, IF, RIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, HEK-293T cells, HepG2 cells, HeLa cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information U2AF2, also named as U2 auxiliary factor 65 kDa subunit , is a 475 amino acid protein, which belongs to the splicing factor SR family. U2AF2 is Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), pre-mRNA, may couple transcription to splicing. The calculated molecular weight of U2AF2 is 53 kDa, but modified U2AF2 is about 65 kDa. Specification Tested Reactivity: human Cited Reactivity: human, mouse, bovine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8070 Product name: Recombinant human U2AF2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 116-471 aa of BC008740 Sequence: IPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW Predict reactive species Full Name: U2 small nuclear RNA auxiliary factor 2 Calculated Molecular Weight: 54 kDa Observed Molecular Weight: 60-65 kDa GenBank Accession Number: BC008740 Gene Symbol: U2AF2 Gene ID (NCBI): 11338 RRID: AB_2211330 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P26368 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.