Proteintech, 15636-1-AP, RTN4IP1 Polyclonal antibody

Size: 20ul / 150ul The RTN4IP1 (15636-1-AP) by Proteintech is a Polyclonal antibody targeting RTN4IP1 in WB, IF/ICC, ELISA applications with reactivity to human samples 15636-1-AP targets RTN4IP1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, HeLa cells, Jurkat cells, MCF-7 cells Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information RTN4IP1 (Reticulon-4-interacting protein 1, mitochondrial), also known as NIMP. It colocalizes with the endoplasmic reticulum HSPA5 at spots corresponding to contacts with mitochondria (PMID: 26593267), which is widely expressed in mitochondria-enriched tissues. Found in heart, muscle, kidney, liver, brain and placenta (PMID: 12067236). The protein plays a role in the regulation of retinal ganglion cell (RGC) neurite outgrowth, and hence in the development of the inner retina and optic nerve. Appears to be a potent inhibitor of regeneration following spinal cord injury. The protein has three isoforms with molecular weights of 43 kDa, 32 kDa and 24 kDa respectively. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8100 Product name: Recombinant human RTN4IP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-396 aa of BC006399 Sequence: MEFLKTCVLRRNACTAVCFWRSKVVQKPSVRRISTTSPRSTVMPAWVIDKYGKNEVLRFTQNMMMPIIHYPNEVIVKVHAASVNPIDVNMRSGYGATALNMKRDPLHVKIKGEEFPLTLGRDVSGVVMECGLDVKYFKPGDEVWAAVPPWKQGTLSEFVVVSGNEVSHKPKSLTHTQAASLPYVALTAWSAINKVGGLNDKNCTGKRVLILGASGGVGTFAIQVMKAWDAHVTAVCSQDASELVRKLGADDVIDYKSGSVEEQLKSLKPFDFILDNVGGSTETWAPDFLKKWSGATYVTLVTPFLLNMDRLGIADGMLQTGVTVGSKALKHFWKGVHYRWAFFMASGPCLDDIAELVDAGKIRPVIEQTFPFSKVPEAFLKVERGHARGKTVINVV Predict reactive species Full Name: reticulon 4 interacting protein 1 Calculated Molecular Weight: 396 aa, 44 kDa Observed Molecular Weight: 32-44 kDa, 24 kDa GenBank Accession Number: BC006399 Gene Symbol: RTN4IP1 Gene ID (NCBI): 84816 RRID: AB_3669217 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8WWV3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.