Proteintech, 15647-1-AP, PDK2 Polyclonal antibody

Size: 20ul / 150ul The PDK2 (15647-1-AP) by Proteintech is a Polyclonal antibody targeting PDK2 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples 15647-1-AP targets PDK2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human heart tissue, HeLa cells, mouse skeletal muscle tissue, NCI-H1299 cells, rat heart tissue Positive IP detected in: mouse skeletal muscle tissue Positive IHC detected in: mouse brain tissue, human brain tissue, human testis tissue, human placenta tissue, human kidney tissue, human heart tissue, human liver tissue, human skin tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2400 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:100-1:600 Background Information PDK2([Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial) is also named as PDHK2 and belongs to the PDK/BCKDK protein kinase family. PDK2 plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. PDK2 is also involved in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. This protein has 2 isoforms produced by alternative splicing. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8183 Product name: Recombinant human PDK2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 135-407 aa of BC005811 Sequence: LEYKDTYGDDPVSNQNIQYFLDRFYLSRISIRMLINQHTLIFDGSTNPAHPKHIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINAANSKQPIHMVYVPSHLYHMLFELFKNAMRATVESHESSLILPPIKVMVALGEEDLSIKMSDRGGGVPLRKIERLFSYMYSTAPTPQPGTGGTPLAGFGYGLPISRLYAKYFQGDLQLFSMEGFGTDAVIYLKALSTDSVERLPVYNKSAWRHYQTIQEAGDWCVPSTEPKNTSTYRVS Predict reactive species Full Name: pyruvate dehydrogenase kinase, isozyme 2 Calculated Molecular Weight: 407 aa, 46 kDa Observed Molecular Weight: 46 kDa GenBank Accession Number: BC005811 Gene Symbol: PDK2 Gene ID (NCBI): 5164 RRID: AB_2268006 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q15119 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.