Product Description
Size: 20ul / 150ul
The RAB14 (15662-1-AP) by Proteintech is a Polyclonal antibody targeting RAB14 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples
15662-1-AP targets RAB14 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications
Positive WB detected in: mouse brain tissue, mouse kidney tissue, HepG2 cells, Raji cells, human brain tissue
Positive IHC detected in: human ovary tumor tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Recommended dilution
Western Blot (WB): WB : 1:500-1:1000
Immunohistochemistry (IHC): IHC : 1:50-1:500
Background Information
RAB14, the final member of the Rab11 subfamily, participates in the regulation of cell secretion, ensuring the timely release of necessary substances outside the cell. RAB14 is ubiquitously expressed and localizes to the Golgi/TGN and at early endosomes (PMID: 16962593, 22595670). RAB14 contains 215 amino acids, with a molecular weight of approximately 24.6 kDa
Specification
Tested Reactivity: human, mouse, rat
Cited Reactivity: human, mouse, pig
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Immunogen: CatNo: Ag8092 Product name: Recombinant human RAB14 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-215 aa of BC006081 Sequence: MATAPYNYSYIFKYIIIGDMGVGKSCLLHQFTEKKFMADCPHTIGVEFGTRIIEVSGQKIKLQIWDTAGQERFRAVTRSYYRGAAGALMVYDITRRSTYNHLSSWLTDARNLTNPNTVIILIGNKADLEAQRDVTYEEAKQFAEENGLLFLEASAKTGENVEDAFLEAAKKIYQNIQDGSLDLNAAESGVQHKPSAPQGGRLTSEPQPQREGCGC Predict reactive species
Full Name: RAB14, member RAS oncogene family
Calculated Molecular Weight: 215 aa, 24 kDa
Observed Molecular Weight: 24 kDa
GenBank Accession Number: BC006081
Gene Symbol: RAB14
Gene ID (NCBI): 51552
RRID: AB_2173630
Conjugate: Unconjugated
Form: Liquid
Purification Method: Antigen affinity purification
UNIPROT ID: P61106
Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.
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Collaboration
Tony Tang
Email: Tony.Tang@iright.com
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