Proteintech, 15675-1-AP, CD44 Polyclonal antibody

Size: 20ul / 150ul The CD44 (15675-1-AP) by Proteintech is a Polyclonal antibody targeting CD44 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications with reactivity to human samples 15675-1-AP targets CD44 in WB, IHC, IF/ICC, IF-P, IP, CoIP, Neutralization, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, HeLa cells, HUVEC cells, MDA-MB-231 cells Positive IP detected in: HeLa cells Positive IHC detected in: human lung cancer tissue, human colon cancer tissue, human oesophagus cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human colon cancer tissue Positive IF/ICC detected in: HeLa cells, human colon cancer tissue, A375 cells, HUVEC cells, UCMSCs cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications. Specification Tested Reactivity: human Cited Reactivity: human, bovine, sika deer Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8143 Product name: Recombinant human CD44 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 28-380 aa of BC004372 Sequence: CRFAGVFHVEKNGRYSISRTEAADLCKAFNSTLPTMAQMEKALSIGFETCRYGFIEGHVVIPRIHPNSICAANNTGVYILTSNTSQYDTYCFNASAPPEEDCTSVTDLPNAFDGPITITIVNRDGTRYVQKGEYRTNPEDIYPSNPTDDDVSSGSSSERSSTSGGYIFYTFSTVHPIPDEDSPWITDSTDRIPATSTSSNTISAGWEPNEENEDERDRHLSFSGSGIDDDEDFISSTISTTPRAFDHTKQNQDWTQWNPSHSNPEVLLQTTTRMTDVDRNGTTAYEGNWNPEAHPPLIHHEHHEEEETPHSTSTIQATPSSTTEETATQKEQWFGNRWHEGYRQTPREDSHST Predict reactive species Full Name: CD44 molecule (Indian blood group) Calculated Molecular Weight: 742 aa, 82 kDa Observed Molecular Weight: 80-95 kDa GenBank Accession Number: BC004372 Gene Symbol: CD44 Gene ID (NCBI): 960 ENSEMBL Gene ID: ENSG00000026508 RRID: AB_2076198 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P16070 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.