Proteintech, 15678-1-AP, CDC45L Polyclonal antibody

Size: 20ul / 150ul The CDC45L (15678-1-AP) by Proteintech is a Polyclonal antibody targeting CDC45L in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 15678-1-AP targets CDC45L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: K-562 cells, HEK-293 cells, HeLa cells, Jurkat cells, mouse thymus tissue Positive IP detected in: K-562 cells Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information CDC45L(Cell division control protein 45 homolog), plays a role in the initiation of DNA replication, and genetically interacts with MCM family members and with ORC2 and physically form a complex including Mcm5p. It's the first gene missed in DiGeorge syndrome that suggest involve in cell proliferation. This is a rabbit polyclonal antibody raised against part of C-terminus of human CDC45L. Specification Tested Reactivity: human, mouse Cited Reactivity: human, chicken Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8150 Product name: Recombinant human CDC45L protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 217-566 aa of BC010022 Sequence: LWWAIVGLTDQWVQDKITQMKYVTDVGVLQRHVSRHNHRNEDEENTLSVDCTRISFEYDLRLVLYQHWSLHDSLCNTSYTAARFKLWSVHGQKRLQEFLADMGLPLKQVKQKFQAMDISLKENLREMIEESANKFGMKDMRVQTFSIHFGFKHKFLASDVVFATMSLMESPEKDGSGTDHFIQALDSLSRSNLDKLYHGLELAKKQLRATQQTIASCLCTNLVISQGPFLYCSLMEGTPDVMLFSRPASLSLLSKHLLKSFVCSTKNRRCKLLPLVMAAPLSMEHGTVTVVGIPPETDSSDRKNFFGRAFEKAAESTSSRMLHNHFDLSVIELKAEDRSKFLDALISLLS Predict reactive species Full Name: CDC45 cell division cycle 45-like (S. cerevisiae) Calculated Molecular Weight: 64 kDa Observed Molecular Weight: 60-66 kDa GenBank Accession Number: BC010022 Gene Symbol: CDC45L Gene ID (NCBI): 8318 RRID: AB_10642704 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O75419 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.