Proteintech, 15695-1-AP, MAP1S Polyclonal antibody

Size: 20ul / 150ul The MAP1S (15695-1-AP) by Proteintech is a Polyclonal antibody targeting MAP1S in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 15695-1-AP targets MAP1S in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells Positive IP detected in: SH-SY5Y cells Positive IHC detected in: human prostate cancer tissue, human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U2OS cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information MAP1S (also known as C19ORF5 or VCY2IP1) is a novel member of the microtubule-associated protein 1 family and a homologue of the exclusively neuronal distributed microtubule-associated protein 1A and 1B (MAP1A/B). In contrast to MAP1A and MAP1B, MAP1S is expressed in a wide range of tissues in addition to neurons. MAP1S is synthesized as a precursor protein that is partially cleaved into heavy and light chains in a tissue-specific manner. In addition, a short chain isoform may be induced under prolonged mitotic arrest or inhibition of the 26S proteasome. Recently it has been reported that the short chain isoform associates with mitochondria in addition to microtubules and causes irreversible aggregation of dysfunctional mitochondria resulting in cell death. Western blot analysis in human brain using this antibody detected two main bands between 100-130 kDa corresponding to heavy and light chains of MAP1S. Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8315 Product name: Recombinant human MAP1S protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 457-806 aa of BC008806 Sequence: APTSEAGLSLPLRGPRARRSASPHDVDLCLVSPCEFEHRKAVPMAPAPASPGSSNDSSARSQERAGGLGAEETPPTSVSESLPTLSDSDPVPLAPGAADSDEDTEGFGVPRHDPLPDPLKVPPPLPDPSSICMVDPEMLPPKTARQTENVSRTRKPLARPNSRAAAPKATPVAAAKTKGLAGGDRASRPLSARSEPSEKGGRAPLSRKSSTPKTATRGPSGSASSRPGVSATPPKSPVYLDLAYLPSGSSAHLVDEEFFQRVRALCYVISGQDQRKEEGMRAVLDALLASKQHWDRDLQVTLIPTFDSVAMHTWYAETHARHQALGITVLGSNSMVSMQDDAFPACKVEF Predict reactive species Full Name: microtubule-associated protein 1S Calculated Molecular Weight: 806 aa, 85 kDa Observed Molecular Weight: 130-150 kDa GenBank Accession Number: BC008806 Gene Symbol: MAP1S Gene ID (NCBI): 55201 RRID: AB_2137868 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q66K74 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.