Proteintech, 15704-1-AP, EIF4G1 Polyclonal antibody

Size: 20ul / 150ul The EIF4G1 (15704-1-AP) by Proteintech is a Polyclonal antibody targeting EIF4G1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 15704-1-AP targets EIF4G1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: BxPC-3 cells, HeLa cells, HepG2 cells Positive IP detected in: HeLa cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells, Ethacrynic acid treated HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Eukaryotic cellular messenger RNAs are posttranscriptionally modified by addition of an m(7)GTP moiety to the 5-prime terminus, referred to as a cap. Recognition of the cap structure and unwinding of mRNA secondary structure during the initiation phase of protein synthesis is catalyzed by initiation factors of the eIF4 group. EIF4G1, a subunit of eIF4 gamma, forms various complexes with the other eIF4 polypeptides [PMID: 7601469]. Mutations in the EIF4G1 gene, encoding a component of the eIF4F translation initiation complex, were recently reported as a possible cause for the autosomal dominant form of Parkinson's disease [PMID:22658323]. The calcualted molecular weight of EIF4G1 is 175 kDa, but modified EIF4G1 is about 220-240 kDa. (PMID: 18426977 ) Specification Tested Reactivity: human Cited Reactivity: human, mouse, pig Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8342 Product name: Recombinant human EIF4G1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 296-645 aa of BC007788 Sequence: IEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN Predict reactive species Full Name: eukaryotic translation initiation factor 4 gamma, 1 Calculated Molecular Weight: 1600 aa, 176 kDa Observed Molecular Weight: 230-250 kDa GenBank Accession Number: BC007788 Gene Symbol: EIF4G1 Gene ID (NCBI): 1981 RRID: AB_2261979 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q04637 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.