Proteintech, 15731-1-AP, NUF2 Polyclonal antibody

Size: 20ul / 150ul The NUF2 (15731-1-AP) by Proteintech is a Polyclonal antibody targeting NUF2 in WB, IP, ELISA applications with reactivity to human samples 15731-1-AP targets NUF2 in WB, IHC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, HepG2 cells, HeLa cells, human liver tissue, human testis tissue, PC-3 cells, human skeletal muscle tissue Positive IP detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information NUF2 (NUF2, Ndc80 kinetochore complex component) plays an important role in kinetochore-microtubule attachment. NUF2 was reported to be overexpressed in various kinds of carcinomas including lung cancer, cholangiocellular cancer, urinary bladder cancer, renal cell cancer, colorectal cancer, gastric cancer, ovarian cancer and serous adenocarcinoma. Elevated expression of NUF2 was associated with poorer prognosis of non-small cell lung cancer patients (PMID: 17079454). Knockdown of NUF2 significantly caused cell growth delay and increased apoptosis in cell lines of non-small cell lung cancer, colorectal cancer, gastric cancer and ovarian cancer. Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8479 Product name: Recombinant human NUF2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 105-464 aa of BC008489 Sequence: ETADILCPKAKRTSRFLSGIINFIHFREACRETYMEFLWQYKSSADKMQQLNAAHQEALMKLERLDSVPVEEQEEFKQLSDGIQELQQSLNQDFHQKTIVLQEGNSQKKSNISEKTKRLNELKLSVVSLKEIQESLKTKIVDSPEKLKNYKEKMKDTVQKLKNARQEVVEKYEIYGDSVDCLPSCQLEVQLYQKKIQDLSDNREKLASILKESLNLEDQIESDESELKKLKTEENSFKRLMIVKKEKLATAQFKINKKHEDVKQYKRTVIEDCNKVQEKRGAVYERVTTINQEIQKIKLGIQQLKDAAEREKLKSQEIFLNLKTALEKYHDGIEKAAEDSYAKIDEKTAELKRKMFKMST Predict reactive species Full Name: NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae) Calculated Molecular Weight: 464 aa, 54 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC008489 Gene Symbol: NUF2 Gene ID (NCBI): 83540 RRID: AB_2154586 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BZD4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.