Proteintech, 15743-1-AP, CLPB Polyclonal antibody

Size: 20ul / 150ul The CLPB (15743-1-AP) by Proteintech is a Polyclonal antibody targeting CLPB in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 15743-1-AP targets CLPB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: COLO 320 cells, HeLa cells, Jurkat cells, K-562 cells Positive IHC detected in: human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:100-1:400 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information CLPB encodes a protein of 707 aa with a predicted molecular mass of approximately 79 kDa, presenting two conserved ATP-binding domains. CLPB is a member of the AAA+ protein family. This potential internal translation initiation site suggests that a smaller form of ClpB, with a theoretical molecular mass of approximately 58 kDa, may be produced. (PMID: 25597510, PMID: 255957260) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8165 Product name: Recombinant human CLPB protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 359-707 aa of BC006404 Sequence: AAIRRKENGWYDEEHPLVFLFLGSSGIGKTELAKQTAKYMHKDAKKGFIRLDMSEFQERHEVAKFIGSPPGYVGHEEGGQLTKKLKQCPNAVVLFDEVDKAHPDVLTIMLQLFDEGRLTDGKGKTIDCKDAIFIMTSNVASDEIAQHALQLRQEALEMSRNRIAENLGDVQISDKITISKNFKENVIRPILKAHFRRDEFLGRINEIVYFLPFCHSELIQLVNKELNFWAKRAKQRHNITLLWDREVADVLVDGYNVHYGARSIKHEVERRVVNQLAAAYEQDLLPGGCTLRITVEDSDKQLLKSPELPSPQAEKRLPKLRLEIIDKDSKTRRLDIRAPLHPEKVCNTI Predict reactive species Full Name: ClpB caseinolytic peptidase B homolog (E. coli) Calculated Molecular Weight: 707 aa, 79 kDa Observed Molecular Weight: 58 kDa GenBank Accession Number: BC006404 Gene Symbol: CLPB Gene ID (NCBI): 81570 RRID: AB_2847900 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9H078 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.