Proteintech, 15749-1-AP, DOK3 Polyclonal antibody

Size: 20ul / 150ul The DOK3 (15749-1-AP) by Proteintech is a Polyclonal antibody targeting DOK3 in WB, ELISA applications with reactivity to human, mouse, rat samples 15749-1-AP targets DOK3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: K-562 cells, Ramos cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Background Information The adaptor proteins downstream of kinase 1 (DOK1) to DOK3 are mainly found in immune cells, and they are closely related, with some functional redundancy, although each DOK protein has distinct properties and functions. DOK1 to DOK3 inhibit LPS responses. DOK proteins have an N-terminal pleckstrin homology (PH) domain, a phosphotyrosine-binding (PTB) domain, and four tyrosine-containing motifs that can bind to Src homology 2 (SH2) domains when phosphorylated. DOK3 was also shown to have an inhibitory role as it restricts Ca2+ and JNK activation in B cell receptor signaling. The MW of this protein is 53 kDa, and this antibody specially recognises the 53 kDa protein. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8221 Product name: Recombinant human DOK3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-330 aa of BC004564 Sequence: MDPLETPIKDGILYQQHVKFGKKCWRKVWALLYAGGPSGVARLESWEVRDGGLGAAGDRSAGPGRRGERRVIRLADCVSVLPADGESCPRDTGAFLLTTTERSHLLAAQHRQAWMGPICQLAFPGTGEASSGSTDAQSPKRGLVPMEENSIYSSWQEVGEFPVVVQRTEAATRCQLKGPALLVLGPDAIQLREAKGTQALYSWPYHFLRKFGSDKILLGTPGVSLLICKGERTDDVSGIILDESLLRAYSVPGAGGHSRVQDSLGPVLREPTFQGERSFLKTSMLRSLLCSCSWRHPRSQPCTQASCLQGSDCPAPHRNSTSAAHTLGTS Predict reactive species Full Name: docking protein 3 Calculated Molecular Weight: 496aa,53 kDa; 330aa,36 kDa Observed Molecular Weight: 53 kDa GenBank Accession Number: BC004564 Gene Symbol: DOK3 Gene ID (NCBI): 79930 RRID: AB_2095099 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q7L591 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.