Proteintech, 15773-1-AP, VAX2 Polyclonal antibody

Size: 20ul / 150ul The VAX2 (15773-1-AP) by Proteintech is a Polyclonal antibody targeting VAX2 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples 15773-1-AP targets VAX2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse eye tissue Positive IP detected in: Y79 cells Positive IHC detected in: human ovary tumor tissue, rat eye tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information Ventral anterior homeobox 2 (VAX2) has been reported to be specifically expressed in the ventral region of the prospective neural retina in vertebrates and plays crucial roles in regulating the proper development of the ventral region of eye (PMID: 21148184). VAX2 inactivatiin mouse leads to alterations in the establishment of a proper dorsoventral eye axis during embryonic development (PMID: 33817157). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8439 Product name: Recombinant human VAX2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-290 aa of BC006336 Sequence: MGDGGAERDRGPARRAESGGGGGRCGDRSGAGDLRADGGGHSPTEVAGTSASSPAGSRESGADSDGQPGPGEADHCRRILVRDAKGTIREIVLPKGLDLDRPKRTRTSFTAEQLYRLEMEFQRCQYVVGRERTELARQLNLSETQVKVWFQNRRTKQKKDQSRDLEKRASSSASEAFATSNILRLLEQGRLLSVPRAPSLLALTPSLPGLPASHRGTSLGDPRNSSPRLNPLSSASASPPLPPPLPAVCFSSAPLLDLPAGYELGSSAFEPYSWLERKVGSASSCKKANT Predict reactive species Full Name: ventral anterior homeobox 2 Calculated Molecular Weight: 290 aa, 31 kDa Observed Molecular Weight: 31 kDa GenBank Accession Number: BC006336 Gene Symbol: VAX2 Gene ID (NCBI): 25806 RRID: AB_2878183 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UIW0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.