Proteintech, 15817-1-AP, XRCC4 Polyclonal antibody

Size: 20ul / 150ul The XRCC4 (15817-1-AP) by Proteintech is a Polyclonal antibody targeting XRCC4 in WB, IHC, IP, ELISA applications with reactivity to human, rat samples 15817-1-AP targets XRCC4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: HepG2 cells, HEK-293 cells, COLO 320 cells Positive IP detected in: HepG2 cells Positive IHC detected in: human breast cancer tissue, rat testis tissue, rat small intestine tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). XRCC4 is one of the core machiney of NHEJ that required for double-strand break repair and V(D)J recombination. The DNA ligase IV(LIG4)-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Specification Tested Reactivity: human, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8606 Product name: Recombinant human XRCC4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 6-310 aa of BC005259 Sequence: SRIHLVSEPSITHFLQVSWEKTLESGFVITLTDGHSAWTGTVSESEISQEADDMAMEKGKYVGELRKALLSGAGPADVYTFNFSKESCYFFFEKNLKDVSFRLGSFNLEKVENPAEVIRELICYCLDTIAENQAKNEHLQKENERLLRDWNDVQGRFEKCVSAKEALETDLYKRFILVLNEKKTKIRSLHNKLLNAAQEREKDIKQEGETAICSEMTADRDPVYDESTDEESENQTDLSGLASAAVSKDDSIISSLDVTDIAPSRKRRQRMQRNLGTEPKMAPQENQLQEKEKGRKKETSEKEAV Predict reactive species Full Name: X-ray repair complementing defective repair in Chinese hamster cells 4 Calculated Molecular Weight: 310 aa, 35 kDa Observed Molecular Weight: 55 kDa GenBank Accession Number: BC005259 Gene Symbol: XRCC4 Gene ID (NCBI): 7518 RRID: AB_2257478 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q13426 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.