Proteintech, 15823-1-AP, UBASH3A-Specific Polyclonal antibody

Size: 20ul / 150ul The UBASH3A-Specific (15823-1-AP) by Proteintech is a Polyclonal antibody targeting UBASH3A-Specific in WB, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 15823-1-AP targets UBASH3A-Specific in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse bone marrow Positive IP detected in: mouse spleen tissue Positive IF/ICC detected in: THP-1 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information UBASH3A gene encodes ubiquitin-associated and SH3 domain-containing protein A (UBASH3A) also known as T-cell ubiquitin ligand 1 (TULA1) which is one of two family members belonging to the T-cell ubiquitin ligand (TULA) family. UBASH3A gene expression is limited to only a few tissues, with its highest expression in spleen, peripheral blood leukocytes, and bone marrow. UBASH3A protein is associated with c-Cbl and ubiquitylated proteins and has been implicated in the regulation of signaling mediated by protein-tyrosine kinases oth family members can negatively regulate T-cell signaling. UBASH3A can facilitate growth factor withdrawal-induced apoptosis in T cells via its interaction with apoptosis-inducing factor (AIF). This antibody is UBASH3A-specific. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: ubiquitin associated and SH3 domain containing, A Calculated Molecular Weight: 74 kDa Observed Molecular Weight: 70-74 kDa GenBank Accession Number: NM_018961 Gene Symbol: UBASH3A Gene ID (NCBI): 53347 RRID: AB_2272584 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P57075 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.