Proteintech, 15872-1-AP, FABP4 Polyclonal antibody

Size: 20ul / 150ul The FABP4 (15872-1-AP) by Proteintech is a Polyclonal antibody targeting FABP4 in WB, IHC, IF, ELISA applications with reactivity to human, mouse, rat samples 15872-1-AP targets FABP4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human adipose tissue, MC38 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue, mouse adipose tissue, mouse brown adipose tissue, mouse heart tissue, rat adipose tissue Positive IHC detected in: human colon tissue, human cervix tissue, human skin tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF detected in: mouse adipose, HUVEC cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF): IF : 1:50-1:500 Background Information Fatty acid binding protein (FABP) 4 is a member of the FABP family which abundantly expressed, fatty acid carrier proteins. FABPs are capable of binding a variety of hydrophobic molecules such as long-chain fatty acids and are important for their uptake and intracellular trafficking. It was first identified as an adipocyte-specific protein, important for the maintenance of lipid and glucose metabolism. It is also detected in macrophages, where it participates in regulating inflammation and cholesterol trafficking via NFκB and PPAR. In more recent studies, FABP4 has been found in a variety of endothelial cells, where it has been identified as a target of VEGF and a regulator of cell proliferation and possibly angiogenesis. Pathologically, FABP4 has been associated with the development of metabolic syndrome, diabetes and cancer and vulnerability of atherosclerotic plaques. FABP4 has been identified as a novel prognostic factor for both adverse cardiovascular events and breast cancer. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8631 Product name: Recombinant human FABP4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 6-132 aa of BC003672 Sequence: VGTWKLVSSENFDDYMKEVGVGFATRKVAGMAKPNMIISVNGDVITIKSESTFKNTEISFILGQEFDEVTADDRKVKSTITLDGGVLVHVQKWDGKSTTIKRKREDDKLVVECVMKGVTSTRVYERA Predict reactive species Full Name: fatty acid binding protein 4, adipocyte Calculated Molecular Weight: 132 aa, 15 kDa Observed Molecular Weight: 15 kDa GenBank Accession Number: BC003672 Gene Symbol: FABP4 Gene ID (NCBI): 2167 RRID: AB_2102440 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P15090 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.