Proteintech, 15921-1-AP, ERCC8 Polyclonal antibody

Size: 20ul / 150ul The ERCC8 (15921-1-AP) by Proteintech is a Polyclonal antibody targeting ERCC8 in WB, ELISA applications with reactivity to human, mouse samples 15921-1-AP targets ERCC8 in WB, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HL-60 cells, HepG2 cells, RAW 264.7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Background Information ERCC8 (also known as Cockayne Syndrome A protein, CSA) is a critical nuclear protein that serves as a specific adapter component of the CRL4ᴱᴿᶜᶜ⁸ ubiquitin ligase complex. Its primary function is to play a central role in the transcription-coupled nucleotide excision repair pathway, specifically recognizing and repairing the template strands of actively transcribed genes damaged by factors such as ultraviolet radiation. Mutations in the ERCC8 gene cause Cockayne syndrome, an autosomal recessive disorder characterized by severe photosensitivity, premature aging, neurodevelopmental abnormalities, and sensorineural hearing loss. This demonstrates that ERCC8 is essential for maintaining transcriptional fidelity, neuronal survival, and combating oxidative stress. Therefore, ERCC8 is not only a key factor in DNA repair but also an important molecule for studying mechanisms of premature aging and neurobiology. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8734 Product name: Recombinant human ERCC8 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-205 aa of BC009793 Sequence: MLGFLSARQTGLEDPLRLRRAESTRRVLGLELNKDRDVERIHGGGINTLDIEPVEGRYMLSGGSDGVIVLYDLENSSRQSYYTCKAVCSIGRDHPDVHRYSVETVQWYPHDTGMFTSSSFDKTLKVWDTNTLQTADVFNFEETVYSHHMSPVSTKHCLVAVGTRGPKVQLCDLKSGSCSHILQGIFILFQTATTLSKRFNKKKRY Predict reactive species Full Name: excision repair cross-complementing rodent repair deficiency, complementation group 8 Calculated Molecular Weight: 396 aa, 44 kDa Observed Molecular Weight: 42 kDa GenBank Accession Number: BC009793 Gene Symbol: ERCC8 Gene ID (NCBI): 1161 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q13216 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.