Proteintech, 15958-1-AP, SEC24A Polyclonal antibody

Size: 20ul / 150ul The SEC24A (15958-1-AP) by Proteintech is a Polyclonal antibody targeting SEC24A in WB, IHC, ELISA applications with reactivity to human, mouse, rat, monkey samples 15958-1-AP targets SEC24A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples. Tested Applications Positive WB detected in: COS-7 cells, HeLa cells, human liver tissue, mouse skeletal muscle tissue, mouse thymus tissue, rat thymus tissue, HEK-293 cells, HepG2 cells, NIH/3T3 cells Positive IHC detected in: human lymphoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445). Two isoforms of 120 kDa and 66 kDa produced by alternative splicing have been described. Specification Tested Reactivity: human, mouse, rat, monkey Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8723 Product name: Recombinant human SEC24A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 264-613 aa of BC019341 Sequence: DEIEGGGLLATPQLTNKNPKMSRSVGYSYPSLPPGYQNITPPGATGVPPSSLNYPSGPQAFTQTPLGANHLTTSMSGLSLQPEGLRVVNLLQERNMLPSTPLKPPVPNLHEDIQKLNCNPELFRCTLTSIPQMQALLNKAKLPLGLLLHPFKDLVQLPVVTSSTIVRCRSCRTYINPFVSFLDQRRWKCNLCYRVNDVPEEFLYNPLTRVYGEPHRRPEVQNATIEFMAPSEYMLRPPQPPVYLFVFDVSHNAVETGYLNSVCQSLLDNLDLLPGNTRTKIGFITFDSTIHFYGLQESLSQPQMLIVSDIEDVFIPMPENLLVNLNESKESVIGVSSEETLITCLEIAMR Predict reactive species Full Name: SEC24 family, member A (S. cerevisiae) Calculated Molecular Weight: 613 aa, 66 kDa Observed Molecular Weight: 66 kDa, 120 kDa GenBank Accession Number: BC019341 Gene Symbol: SEC24A Gene ID (NCBI): 10802 RRID: AB_2186118 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O95486 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.