Proteintech, 15972-1-AP, ACOT7 Polyclonal antibody

Size: 20ul / 150ul The ACOT7 (15972-1-AP) by Proteintech is a Polyclonal antibody targeting ACOT7 in WB, ELISA applications with reactivity to human, mouse, rat samples 15972-1-AP targets ACOT7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293T cells, mouse brain tissue, human brain tissue, Jurkat cells, rat brain tissue Recommended dilution Western Blot (WB): WB : 1:3000-1:10000 Background Information Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:10578051).  Acyl-coenzyme A thioesterase 7/ACOT7 preferentially hydrolyzes palmitoyl-CoA, but has a broad specificity acting on other fatty acyl-CoAs with chain-lengths of C8-C18 (PubMed:10578051). May play an important physiological function in brain (PubMed:10578051). ACOT7 has 7 isoforms between 27-40 kDa, we can detect a band at 37 kDa. ACOT7 is high homology with ACOT7L, this antibody may also detect ACOT7L. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, bovine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8759 Product name: Recombinant human ACOT7 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 14-370 aa of BC017365 Sequence: PDTCALLQPPAASAAAAPSMSGPDVETPSAIQICRIMRPDDANVAGNVHGGTILKMIEEAGAIISTRHCNSQNGERCVAALARVERTDFLSPMCIGEVAHVSAEITYTSKHSVEVQVNVMSENILTGAKKLTNKATLWYVPLSLKNVDKVLEVPPVVYSRQEQEEEGRKRYEAQKLERMETKWRNGDIVQPVLNPEPNTVSYSQSSLIHLVGPSDCTLHGFVHGGVTMKLMDEVAGIVAARHCKTNIVTASVDAINFHDKIRKGCVITISGRMTFTSNKSMEIEVLVDADPVVDSSQKRYRAASAFFTYVSLSQEGRSLPVPQLVPETEDEKKRFEEGKGRYLQMKAKRQGHAEPQP Predict reactive species Full Name: acyl-CoA thioesterase 7 Calculated Molecular Weight: 370 aa, 40 kDa Observed Molecular Weight: 37 kDa GenBank Accession Number: BC017365 Gene Symbol: ACOT7 Gene ID (NCBI): 11332 RRID: AB_2221652 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O00154 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.