Proteintech, 16010-1-AP, LAS1L Polyclonal antibody

Size: 20ul / 150ul The LAS1L (16010-1-AP) by Proteintech is a Polyclonal antibody targeting LAS1L in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 16010-1-AP targets LAS1L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: COLO 320 cells, HEK-293 cells, HeLa cells, HepG2 cells, PC-3 cells Positive IP detected in: HEK-293 cells Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information LAS1L is a nucleolar protein required for the synthesis of the 60S ribosomal subunit and maturation of the 28S rRNA. LAS1L interacts with PELP1, TEX10, and WDR18, along with NOL9 and SENP3, to form a novel nucleolar complex that cofractionates with the 60S preribosomal subunit. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8732 Product name: Recombinant human LAS1L protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-353 aa of BC014545 Sequence: MSWESGAGPGLGSQGMDLVWSAWYGKCVKGKGSLPLSAHGIVVAWLSRAEWDQVTVYLFCDDHKLQRYALNRITVWRSRSGNELPLAVASTADLIRCKLLDVTGGLGTDELRLLYGMALVRFVNLISERKTKFAKVPLKCLAQEVNIPDWIVDLRHELTHKKMPHINDCRRGCYFVLDWLQKTYWCRQLENSLRETWELEEFREGIEEEDQEEDKNIVVDDITEQKPEPQDDGKSTESDVKADGDSKGSEEVDSHCKKALSHKELYERARELLVSYEEEQFTVLEKFRYLPKAIKAWNNPSPRVECVLAELKGVTCENREAVLDAFLDDGFLVPTFEQLAALQIEYEDGQTEV Predict reactive species Full Name: LAS1-like (S. cerevisiae) Calculated Molecular Weight: 734 aa, 83 kDa Observed Molecular Weight: 83 kDa, 70-75 kDa GenBank Accession Number: BC014545 Gene Symbol: LAS1L Gene ID (NCBI): 81887 RRID: AB_2132810 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y4W2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.