Proteintech, 16108-1-AP, ERGIC1 Polyclonal antibody

Size: 20ul / 150ul The ERGIC1 (16108-1-AP) by Proteintech is a Polyclonal antibody targeting ERGIC1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 16108-1-AP targets ERGIC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, mouse liver tissue, L02 cells Positive IP detected in: HepG2 cells Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information The function of ERGIC1 remains largely unknown. ERGIC1 was highly expressed in most primary prostate tumors. It is an intriguing potential drug target especially for the ERG oncogene expressing tumors (PMID: 22761906). The MW of this protein is 32 kDa, and this antibody specially recognises the 32 kDa protein. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9025 Product name: Recombinant human ERGIC1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 49-290 aa of BC012766 Sequence: TTEVVNELYVDDPDKDSGGKIDVSLNISLPNLHCELVGLDIQDEMGRHEVGHIDNSMKIPLNNGAGCRFEGQFSINKVPGNFHVSTHSATAQPQNPDMTHVIHKLSFGDTLQVQNIHGAFNALGGADRLTSNPLASHDYILKIVPTVYEDKSGKQRYSYQYTVANKEYVAYSHTGRIIPAIWFRYDLSPITVKYTERRQPLYRFITTICAIIGGTFTVAGILDSCIFTASEAWKKIQLGKMH Predict reactive species Full Name: endoplasmic reticulum-golgi intermediate compartment (ERGIC) 1 Calculated Molecular Weight: 290 aa, 33 kDa Observed Molecular Weight: 32 kDa GenBank Accession Number: BC012766 Gene Symbol: ERGIC1 Gene ID (NCBI): 57222 RRID: AB_2098435 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q969X5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.