Proteintech, 16163-1-AP, Presenilin-1-Specific Polyclonal antibody

Size: 20ul / 150ul The Presenilin-1-Specific (16163-1-AP) by Proteintech is a Polyclonal antibody targeting Presenilin-1-Specific in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat, monkey samples 16163-1-AP targets Presenilin-1-Specific in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples. Tested Applications Positive WB detected in: COS-7 cells, HeLa cells, HepG2 cells, Jurkat cells, rat brain tissue, mouse brain tissue Positive IP detected in: mouse kidney tissue Positive IHC detected in: human prostate cancer tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information PSEN1(presenilin-1) is also named as AD3, PS1, PSNL1 and belongs to the peptidase A22A family. It directly influences Netrin/DCC signaling with cell type precision and spatiotemporal specificity, and is a key neural circuit builder that gates the spatiotemporal pattern of guidance signaling, thereby ensuring neural projections occur with high fidelity(PMID:21215373). The 46-kDa PSEN1 holoprotein is cleaved into a D30-kDa N-terminal fragment (NTF) and a D20-kDa C-terminal fragment (CTF) by the 26S proteasome (PMID:10527805). It has 7 isoforms produced by alternative splicing. This antibody is specific to isoform1(53 kDa), isoform2(52 kDa), isoform4(21 kDa), isoform7(49 kDa) of this protein. Specification Tested Reactivity: human, mouse, rat, monkey Cited Reactivity: human, mouse, rat, pig Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: presenilin 1 Calculated Molecular Weight: 53 kDa Observed Molecular Weight: 21 kDa, 53 kDa GenBank Accession Number: NM_000021 Gene Symbol: PSEN1 Gene ID (NCBI): 5663 RRID: AB_2237805 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P49768 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.