Proteintech, 16164-1-AP, HDAC3-specific Polyclonal antibody

Size: 20ul / 150ul The HDAC3-specific (16164-1-AP) by Proteintech is a Polyclonal antibody targeting HDAC3-specific in WB, ELISA applications with reactivity to human samples 16164-1-AP targets HDAC3-specific in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293T cells, HEK-293 cells, human stomach tissue, HeLa cells, HL-60 cells, MOLT-4 cells, NCI-H1299 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Background Information What is the molecular weight of HDAC3? Is HDAC3 post-translationally modified?The molecular weight of HDAC3 is 49 kDa but HDAC3 can form homodimers and homotrimers in cells (PMID: 11779848). HDAC3 can be phosphorylated at S424 residue by protein kinase CK2, which regulates HDAC3 activity (PMID: 15805470).What is the subcellular localization of HDAC3?Unlike other class I HDACs, HDAC3 actively shuttles between the nucleus and cytoplasm (PMID: 11779848). Additionally, HDAC3 can be present at the plasma membrane, where it associates with c-Src (PMID: 16532030).What is the tissue expression pattern of HDAC3?Like other class I HDACs, HDAC3 is ubiquitously expressed (PMID: 9346952).What is the mechanism of HDAC3 in regulating gene expression?Unlike other histone deacetylates, it exists in a complex with two proteins - nuclear receptor co-repressor (N-CoR) and silencing mediator for retinoid and thyroid receptors (SMRT) - and therefore acts as a mediator of the repression function of unliganded nuclear hormone receptors (PMID: 10809664). N-CoR and SMRT play an active role in the recruitment of HDAC3 to binding sites as well as directly regulating HDAC3 activity. Additionally, HDAC3 targets growth-regulated genes. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: histone deacetylase 3 Calculated Molecular Weight: 49 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: NM_003883 Gene Symbol: HDAC3 Gene ID (NCBI): 8841 RRID: AB_2118384 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O15379 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.