Proteintech, 16185-1-AP, ING1-specific Polyclonal antibody

Size: 20ul / 150ul The ING1-specific (16185-1-AP) by Proteintech is a Polyclonal antibody targeting ING1-specific in WB, IP, ELISA applications with reactivity to human, mouse samples 16185-1-AP targets ING1-specific in WB, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: human heart tissue Positive IP detected in: mouse heart tissue Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information Members of inhibitor of growth (ING) family function in inhibiting cell growth and inducing apoptosis. They are sequence homologous proteins. ING1 is the first identified one, interacting with TP53 and is a component of the p53 signaling pathway. Multiple alternatively spliced transcript variants encoding distinct isoforms of ING1 have been reported. This antibody recognizes all 24/33/47kd isoforms of ING1, but will not bind ING2, 3 or 4. Specification Tested Reactivity: human, mouse Cited Reactivity: rat' Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: inhibitor of growth family, member 1 Calculated Molecular Weight: 47 kDa Observed Molecular Weight: 30 kDa GenBank Accession Number: NM_198218 Gene Symbol: ING1 Gene ID (NCBI): 3621 RRID: AB_2233908 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UK53 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.