Proteintech, 16190-1-AP, FcRn-Specific Polyclonal antibody

Size: 20ul / 150ul FcRn-Specific Polyclonal Antibody for WB, IHC, ELISA 16190-1-AP targets FcRn-Specific in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: THP-1 cells, human placenta tissue Positive IHC detected in: human spleen tissue, human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information The neonatal Fc receptor for IgG (FcRn) is a membrane-associated receptor which acts as a key determinant of IgG homeostasis. FcRn is similar in structure to major histocompatibility class I molecules. It is a heterodimer composed of a alpha chain associated with β2-microglobulin. FcRn transfers IgG from the mother to the fetus across the placenta and the proximal small intestine. In addition, throughout life, FcRn protects IgG from degradation and thus regulates the serum half-life of IgG (PMID: 17703228). Specification Tested Reactivity: human Cited Reactivity: human, pig, canine, monkey Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: Fc fragment of IgG, receptor, transporter, alpha Calculated Molecular Weight: 40 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: NM_004107 Gene Symbol: FcRn Gene ID (NCBI): 2217 RRID: AB_2231751 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P55899 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.