Proteintech, 16215-1-AP, ACAT1 Polyclonal antibody

Size: 20ul / 150ul The ACAT1 (16215-1-AP) by Proteintech is a Polyclonal antibody targeting ACAT1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse samples 16215-1-AP targets ACAT1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse heart tissue, Caco-2 cells, human skeletal muscle tissue, MCF-7 cells, rat skeletal muscle tissue, mouse colon tissue Positive IP detected in: mouse heart tissue Positive IHC detected in: human colon, human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information ACAT1 (acetyl-CoA acetyltransferase), is a mitochondrial enzyme catalyzing the reversible formation or breakdown of acetoacetyl-CoA and plays important roles in ketogenesis and ketolysis. Deficiency of ACAT1 causes an inherited metabolic disorder. Recently ACAT1 has been reported to get involved in cancer progression. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat, rabbit Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9215 Product name: Recombinant human ACAT1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-162 aa of BC010942 Sequence: MAVLPALLRSGARSRSPLLRRLVQEIRYVERSYVSKPTLKEVVIVSATRTPIGSFLGSLSLLPATKLGSIAIQGAIEKAGIPKEEVKEAYMGNVLQGGEGQAPTRQAVLGAGLPISTPCTTINKVCASGMKAIMMASQSLMCGHQIKQETGSLAKICCHVRR Predict reactive species Full Name: acetyl-Coenzyme A acetyltransferase 1 Calculated Molecular Weight: 45 kDa Observed Molecular Weight: 38-45 kDa GenBank Accession Number: BC010942 Gene Symbol: ACAT1 Gene ID (NCBI): 38 RRID: AB_2220210 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P24752 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.