Proteintech, 16227-1-AP, ABI3 Polyclonal antibody

Size: 20ul / 150ul The ABI3 (16227-1-AP) by Proteintech is a Polyclonal antibody targeting ABI3 in WB, IHC, ELISA applications with reactivity to human, mouse samples 16227-1-AP targets ABI3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HUVEC cells, SK-N-SH cells Positive IHC detected in: mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information ABI family member 3 belongs to the ABL Interactors (ABI) family of proteins, which also includes ABI1 and ABI2. ABI proteins are a class of cytoplasmic molecular junction proteins originally identified as binding chaperones for Abl kinase. The ABI3 gene is thought to be an important regulator of microglia during Alzheimer's disease (AD), and its S209F variant increases the risk of developing AD. Moreover, ABI3 plays an important role in suppressing tumor growth and progression. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8164 Product name: Recombinant human ABI3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-366 aa of BC007780 Sequence: MAELQQLQEFEIPTGREALRGNHSALLRVADYCEDNYVQATDKRKALEETMAFTTQALASVAYQVGNLAGHTLRMLDLQGAALRQVEARVSTLGQMVNMHMEKVARREIGTLATVQRLPPGQKVIAPENLPPLTPYCRRPLNFGCLDDIGHGIKDLSTQLSRTGTLSRKSIKAPATPASATLGRPPRIPEPVHLPVVPDGRLSAASSASSLASAGSAEGVGGAPTPKGQAAPPAPPLPSSLDPPPPPAAVEVFQRPPTLEELSPPPPDEELPLPLDLPPPPPLDGDELGLPPPPPGFGPDEPSWVPASYLEKVVTLYPYTSQKDNELSFSEGTVICVTRRYSDGWCEGVSSEGTGFFPGNYVEPSC Predict reactive species Full Name: ABI family, member 3 Calculated Molecular Weight: 366 aa, 39 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC007780 Gene Symbol: ABI3 Gene ID (NCBI): 51225 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9P2A4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.